Application
NFkB p100 antibody [C2C3], C-term detects NFkB p100 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p100 antibody [C2C3], C-term diluted at 1:1000.Application
NFkB p100/p52 antibody immunoprecipitates NFkB p100/p52 protein in IP experiments. IP Sample: Jurkat whole cell lysate/extract A. 30 μg Jurkat whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of NFkB p100/p52 protein by 2 μg of NFkB p100/p52 antibody 5% SDS-PAGE The immunoprecipitated NFkB p100/p52 protein was detected by NFkB p100/p52 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
Wild-type (WT) and NFkB p100 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p100 antibody [C2C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 7.5% SDS PAGE NFkB p100 antibody diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000| Product Name | NFkB p100 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NFkB p100 (nuclear factor of kappa light polypeptide gene enhancer in B-cells 2)NFKB has been detected in numerous cell types that express cytokines, chemokines, growth factors, cell adhesion molecules, and some acute phase proteins in health and in various disease states. NFKB is activated by a wide variety of stimuli such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. Inappropriate activation of NF-kappa-B has been linked to inflammatory events associated with autoimmune arthritis, asthma, septic shock, lung fibrosis, glomerulonephritis, atherosclerosis, and AIDS. In contrast, complete and persistent inhibition of NF-kappa-B has been linked directly to apoptosis, inappropriate immune cell development, and delayed cell growth. For reviews, see Chen et al. (1999) [PubMed 9895331] and Baldwin (1996) [PubMed 8717528].[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human NFkB p100. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (90%), Bovine (85%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | LYT-10 antibody LYT10 antibody p52 antibody NFKB2 antibody nuclear factor NF-kappa-B p100 subunit antibody nuclear factor of kappa light chain gene enhancer in B-cells 2 antibody oncogene Lyt-10 antibody DNA-binding factor KBF2 antibody lymphocyte |
|---|---|
| Molecular Weight(MW) | 97 kDa (note) (4) |
| Tissue Specificity | specifically recognizes NFkB p100 isoform, but not the cleaved p52 isoform. |
| Cellular Localization | Nucleus , Cytoplasm |
Application
NFkB p100 antibody [C2C3], C-term detects NFkB p100 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p100 antibody [C2C3], C-term diluted at 1:1000.
Application
NFkB p100/p52 antibody immunoprecipitates NFkB p100/p52 protein in IP experiments. IP Sample: Jurkat whole cell lysate/extract A. 30 μg Jurkat whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of NFkB p100/p52 protein by 2 μg of NFkB p100/p52 antibody 5% SDS-PAGE The immunoprecipitated NFkB p100/p52 protein was detected by NFkB p100/p52 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
Wild-type (WT) and NFkB p100 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p100 antibody [C2C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 7.5% SDS PAGE NFkB p100 antibody diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000| Positive Control | A431 , HeLa , HepG2 , NIH-3T3 , *YY-8103 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
