Application
NFkappaB [pS529] phosphospecific antibody. Jurkat cells unstimulated (1) or stimulated with PMA and Ca2+ iono. (2-5). Lysates incubated with anti-NFkappaB [pS529] after prior incubation with: no peptide (1, 2), the non-phosphopeptide (3), a generic [pS] peptide (4), or the phosphopeptide immunogen (5). Data show antibody phospho-site specificity, and induction of phospho signal after PMA and Ca2+ ionophore treatment| Product Name | NFkB p105 (phospho Ser529) antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to NFkB p50 (phospho Ser529)NFkB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. NF-kB is a family of transcription factors that consists of homo- and heterodimers of NFkB1/p50 and RelA/p65 subunits, and controls a variety of cellular events including development and immune responses. All members share a conserved amino-terminus domain that includes dimerization, nuclear localization, and DNA binding regions, and a carboxy-terminal transactivation domain. Serines 529 and 536 in the transactivation domain of RelA/p65 are phosphorylated in response to several stimuli including phorbol ester, IL-1alpha and TNF-alpha as mediated by IkB kinase and p38 MAPK. Serine 529 is located in a negatively charged region (amino acids 422-540) that is phosphorylated in response to phorbol myristate acetate plus calcium ionophore activation. Phosphorylation of serines 529 and 536 is critical for RelA/p65 transcriptional activity. Activated NF-kB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NF-kB has been associated with a number of inflammatory diseases while persistent inhibition of NF-kB leads to inappropriate immune cell development or delayed cell growth. |
| Immunogen | Synthetic peptide (Human). |
| Modification | p-Ser529 |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/1000. Detects a band of approximately 60 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686C01211 EBP-1 KBF1 MGC54151 NF-kappa-B NF-kappaB NFKB-p105 NFKB-p50 p105 p50 EBP 1 NFKB p50 NF kappaB NFKB p105 NF kappa B EBP1 NFKBp50 NFkappaB NFKBp105 |
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| Target | NFkB p105 (phospho Ser529) |
| Cellular Localization | Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I kappa B). |
Application
NFkappaB [pS529] phosphospecific antibody. Jurkat cells unstimulated (1) or stimulated with PMA and Ca2+ iono. (2-5). Lysates incubated with anti-NFkappaB [pS529] after prior incubation with: no peptide (1, 2), the non-phosphopeptide (3), a generic [pS] peptide (4), or the phosphopeptide immunogen (5). Data show antibody phospho-site specificity, and induction of phospho signal after PMA and Ca2+ ionophore treatment| Application Notes | WB: Use at a dilution of 1/1000. Detects a band of approximately 60 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Form | Liquid |
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| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: 50% Glycerol, 1.0mg/ml BSA, PBS (Ca 2+ and Mg 2+ free). pH 7.3 |
