Application
ELISA analysis of control antigen and antigen using NFKB1 antibody [5D10D11].Application
FACS analysis of MCF-7 cells using NFKB1 antibody [5D10D11] (green) or a negative control (purple).Application
ICC/IF analysis of U251 cells using NFKB1 antibody [5D10D11] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).Application
IHC-P analysis of human intima cancer tissue (left) and human bladder cancer tissue (right) using NFKB1 antibody [5D10D11].Application
WB analysis of (1) K562, (2) Jurkat, (3) A431, (4) HeLa, (5) THP-1 and (6) MCF-7 lysates using NFKB1 antibody [5D10D11].| Product Name | NFkB p105/p50 antibody [5D10D11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody [5D10D11] to NFkB p105/p50This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009] |
| Immunogen | The immunogen corresponding to human NFkB p105/p50 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 5D10D11 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIHC-PWB |
| ELISA: 1/10000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NFKAPPAB , P50 , NFKB-P105 , NF-KAPPA-B , NF-KB1 , EBP-1 , P105 , NF-KAPPAB , KBF1 , NFKB-P50 |
|---|---|
| Target | NFkB p105/p50 |
| Molecular Weight(MW) | 50 kDa/105 kDa (note) |
Application
ELISA analysis of control antigen and antigen using NFKB1 antibody [5D10D11].
Application
FACS analysis of MCF-7 cells using NFKB1 antibody [5D10D11] (green) or a negative control (purple).
Application
ICC/IF analysis of U251 cells using NFKB1 antibody [5D10D11] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).
Application
IHC-P analysis of human intima cancer tissue (left) and human bladder cancer tissue (right) using NFKB1 antibody [5D10D11].
Application
WB analysis of (1) K562, (2) Jurkat, (3) A431, (4) HeLa, (5) THP-1 and (6) MCF-7 lysates using NFKB1 antibody [5D10D11].| Application Notes | ELISA: 1/10000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
