Application
Mixed cultures of rat CNS cells stained with (red) and GeneTex chicken antibody to Microtubule associated protein 2 (MAP2- green, using antibody ). Thea-internexin is localized primarily in neuronal axons in these cultures, while the perikarya and dendrites of neurons stain strongly for MAP2.Application
Left three lanes show Coomassie brilliant blue stained extracts of rat brain stem, cat cerebral cortex and human cerebral cortex (R, C and H respectively). Right three lanes are corresponding blots reacted with stains a band of about 66kDa in all three preparations.| Product Name | NF-L antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [1D2] to NF-LAlpha-internexin is a ~66 kDa Class IV intermediate filament subunit expressed very early in neuronal development. It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. |
| Immunogen | Recombinant full length protein (Rat) expressed in and purified from E. coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | 1D2 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-FIHC-PWB |
| IF: Use at a dilution of 1/500. IHC-Fr: Use at a dilution of 1/5000 (formalin fixed, ABC method). IHC-P: Use at a dilution of 1/5000 (ABC method). WB: Use at a dilution of 1/10000. Detects a band of approximately 66 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanRatMammalian species |
| Concentration | 1mg/ml |
| Gene Synonyms | Nefml Neflmapped Nefl_mapped 68kDa Neurofilament 24589 Nefl |
|---|---|
| Target | NF-L |
Application
Mixed cultures of rat CNS cells stained with (red) and GeneTex chicken antibody to Microtubule associated protein 2 (MAP2- green, using antibody ). Thea-internexin is localized primarily in neuronal axons in these cultures, while the perikarya and dendrites of neurons stain strongly for MAP2.
Application
Left three lanes show Coomassie brilliant blue stained extracts of rat brain stem, cat cerebral cortex and human cerebral cortex (R, C and H respectively). Right three lanes are corresponding blots reacted with stains a band of about 66kDa in all three preparations.| Application Notes | IF: Use at a dilution of 1/500. IHC-Fr: Use at a dilution of 1/5000 (formalin fixed, ABC method). IHC-P: Use at a dilution of 1/5000 (ABC method). WB: Use at a dilution of 1/10000. Detects a band of approximately 66 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 5mM Sodium Azide; Constituents: Tissue Culture Supernatant |
