Application
Immunocytochemistry/Immunofluorescence: 68kDa Neurofilament Light Antibody (DA2) [ - Staining of neurons in culture using NF-L monoclonal DA2 antibody (green) taken with a 40X lens. Nuclei are stained blue.Application
Western Blot: 68kDa Neurofilament Light Antibody (DA2) [ - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibodies including (lane 4).| Product Name | NF-L antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [DA2] to NF-LNeurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-L antibody can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease. |
| Immunogen | NF-L purified from pig spinal cord |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | DA2 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-FIHC-PWB |
| ICC/IF:1:200~1:500 IHC-F:1:50~1:100 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMammalian species |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NF68 NEFL P07196 CMT2E NFL CMT1F NF-L 4747 162280 NF L |
|---|---|
| Target | NF-L |
| Tissue Specificity | Specifically recognizes the light neurofilament subunit or NF-L (~68 kDa). |
| Light Chain Type | kappa |
Application
Immunocytochemistry/Immunofluorescence: 68kDa Neurofilament Light Antibody (DA2) [ - Staining of neurons in culture using NF-L monoclonal DA2 antibody (green) taken with a 40X lens. Nuclei are stained blue.
Application
Western Blot: 68kDa Neurofilament Light Antibody (DA2) [ - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibodies including (lane 4).| Application Notes | ICC/IF:1:200~1:500 IHC-F:1:50~1:100 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Ascitic fluid with 0.1% sodium azide |
