Application
Cells grown from adult rat brain. Large cell in middle is stained with mouse monoclonal to NF-L clone DA2 (green). Another type of neuronal lineage cell was stained with rabbit polyclonal to alpha-internexin RPCa-a-Int (red). These cells were mitotic but had several characteristics of neurons.Application
Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibodies including MCA-DA2 (lane 4).| Product Name | NF-L antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [DA2] to 70 kD Neurofilament Light - Neuronal MarkerNeurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-L antibody can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease. Mutations in the protein coding region of the human NF-L gene appear to cause some forms of Charcot-Marie-Tooth disease. |
| Immunogen | Enzymatically dephosphorylated pig neurofilaments including NF-L, NF-M and NF-H. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | DA2 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| IF: Use at a dilution of 1/100 - 1/500. IHC-Fr: Use at an assay dependant dilution. WB: Use at a dilution of 1/5000 - 1/10000. Predicted molecular weight: 68 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMousePigRatMammalian speciesBovine |
| Concentration | 1mg/ml |
| Target | 70 kDa Neurofilament Light |
|---|---|
| Tissue Specificity | Screening was performed by ELISA using the immunogen, followed by immunofluorescence microscopy. Clones which revealed strong staining were further characterized biochemically. |
| Cellular Localization | Cytoplasmic |
| Light Chain Type | kappa |
Application
Cells grown from adult rat brain. Large cell in middle is stained with mouse monoclonal to NF-L clone DA2 (green). Another type of neuronal lineage cell was stained with rabbit polyclonal to alpha-internexin RPCa-a-Int (red). These cells were mitotic but had several characteristics of neurons.
Application
Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibodies including MCA-DA2 (lane 4).| Application Notes | IF: Use at a dilution of 1/100 - 1/500. IHC-Fr: Use at an assay dependant dilution. WB: Use at a dilution of 1/5000 - 1/10000. Predicted molecular weight: 68 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Constituents: Tissue Culture Supernatant |
