Application
immunofluorescence of neurofilament, light chain in rat cerebral cortex cultures in green.| Product Name | NF-L antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [DA2] to NF-LThe neurofilament class of intermediate filaments is found in high concentrations along the axons of vertebrate neurons. The family consists of three types of proteins: NF-L, NF-M, and NF-H. All three have the conserved tripartite structure of intermediate filaments, but NF-M and NF-L have long, hypervariable C-terminal tail domains that have multiple phosphorylation sites. Neurofilaments have been shown to influence axonal caliber, which is proportional to conduction velocity in mammalian neurons. Amyotrophic lateral sclerosis or Lou Gehrig’s disease has been associated with accumulation and abnormal assembly of neurofilaments in motor neurons and is thought to influence axonal degeneration.NF-L is the central member of the neurofilament family. It has been shown to form the backbone 10nm fiber on which the other neurofilamens assemble. NF-L knockout mice display no axonal NF’s, reduced axonal caliber, and delayed nerve regeneration |
| Immunogen | Enzymatically dephosphorylated porcine neurofilaments. |
| Clonality | Monoclonal |
|---|---|
| Clone Number | DA2 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHC-FIHC-PWB |
| ICC/IF: 1:100: |
|
| Species Reactivity | HumanChickenRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Target | NF-L |
|---|---|
| Tissue Specificity | detects the neurofilament, light chain in human and rat samples. |
Application
immunofluorescence of neurofilament, light chain in rat cerebral cortex cultures in green.| Application Notes | ICC/IF: 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20º C, Avoid Freeze/Thaw Cycles |
| Storage Buffer | 100 ug purified mouse IgG (1 mg/ml) in PBS with 1 mg/ml BSA and 0.05% sodium azide |
