| Product Name | NF-L antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [DA2] to NF-LNeurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-L antibody can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease. |
| Immunogen | NF-L purified from pig spinal cord |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | DA2 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-FIHC-PWB |
| Western blot analysis: 1:500-1:2500 IHC: 1:100-1:500 IF/ICC: 1:100-1:500Can be used on cryostat sections and formalin-fixed paraffin-embedded histological sections, and Western blotting.: |
|
| Species Reactivity | HumanMousePigRatBirdCatMammalian species |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 27255 GTX27255 DA2 70 kD Neurofilament Light - Neuronal Marker 70 kD Neurofilament Light DA2 -Neuronal Marker DA2 70 kD Neurofilament Light Ab7255 DA2 70 kD Neurofilament Light Neuronal Marker 70 kD 68 kDa neurofilament protein antibody NEFL antibody Neuro |
|---|---|
| Target | NF-L |
| Tissue Specificity | Specifically recognizes the light neurofilament subunit or NF-L (~68 kDa). |
| Cellular Localization | Cytoplasmic |
| Light Chain Type | kappa |
| Application Notes | Western blot analysis: 1:500-1:2500 IHC: 1:100-1:500 IF/ICC: 1:100-1:500Can be used on cryostat sections and formalin-fixed paraffin-embedded histological sections, and Western blotting.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | 50% glycerol, 0.01% thimerosal and 1.0 mg/ml BSA. |
