Application
Immunocytochemistry/Immunofluorescence: 160kDa Neurofilament Medium Antibody (3H11) - Culture of adult neural cells grown as described (2). Mature neurons can be identified by their morphology and because they stain strongly with antibodies to NF-L, NF-M and NF-H. The surrounding stellate red cells are stained with rabbit polyclonal Rat facial nucleus before RPCA-a-Int. These are apparently mitotic neuronal progenitor cells and express many other neuronal markersApplication
Western Blot: 160kDa Neurofilament Medium Antibody (3H11) - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibodies including (lane 3).| Product Name | NF-M antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [3H11] to NF-MNeurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-M is the middle or medium neurofilament subunit and runs on SDS-PAGE gels in the range 145-170kDa, with some variation in different species. Antibodies to NF-M are useful to identify this protein and identify neurons and their processes in tissue sections and in tissue culture. NF-M can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease. |
| Immunogen | Recombinant C-terminal fragment (Rat). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3H11 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-FIHC-PWB |
| IHC: Use at a dilution of 1:500. WB: Use at a dilution of 1:2,500. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanRatMammalian species |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Nefm Nfm NEF3 24588 |
|---|---|
| Target | NF-M |
| Tissue Specificity | Specifically recognizes the medium neurofilament subunit (~145-170 kDa). |
| Light Chain Type | kappa |
Application
Immunocytochemistry/Immunofluorescence: 160kDa Neurofilament Medium Antibody (3H11) - Culture of adult neural cells grown as described (2). Mature neurons can be identified by their morphology and because they stain strongly with antibodies to NF-L, NF-M and NF-H. The surrounding stellate red cells are stained with rabbit polyclonal Rat facial nucleus before RPCA-a-Int. These are apparently mitotic neuronal progenitor cells and express many other neuronal markers
Application
Western Blot: 160kDa Neurofilament Medium Antibody (3H11) - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibodies including (lane 3).| Application Notes | IHC: Use at a dilution of 1:500. WB: Use at a dilution of 1:2,500. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
