| Product Name | NGF beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [25623.1] to NGF betaNGF-7S is characterized as a non-covalent aggregate containing three types of polypeptide chains (alpha, beta and gamma). NGFB is isolated from dissociated, homogeneous NGF 7S. The gene encoding the human NGF-has been localized to chromosome 1 (p22.1) by somatic cell hybridization. Comparison of the mouse and human genes indicates that the two genes are organized with similar intron/exon structure. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 25623.1 |
| Host Species | Mouse |
| Tested Applications | DotELISAInhibition |
| Dot: Use at a concentration of 1 - 3 μg/ml - determined using recombinant NGFB at 400 ng under non- and 200 ng under reducing conditions. ELISA: Use at a concentration of 1 - 2 μg/ml (100 μl/well) for capture ELISA, Monoclonal Anti-Human Nerve Growth Factor-Beta can be used as the capture antibody. Determined in combination with a biotinylated NGF-Beta polyclonal detection antibody at 50-100 ng/ml (100 μl/well), an ELISA for sample volumes of 100 μl with a range of 31.2 to 2000 pg/ml may be obtained. For capture ELISA, a working concentration of 1-2 μg/ml is determined to coat plates at 100 μl/well. Inhib: Use at a concentration of 1 - 2 μg/ml. Human NGFB stimulates the 3H-thymidine incorporation by TF-1 cells in a dose-dependent manner. The Neutralization Dose50 (ND50) for anti-human NGFB is approximately 1-3 μg/ml in the presence of 5 ng/ml of recombinant human NGFB using the NGFB responsive cell line TF-1. The ND50 is the concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when the cytokine is present at a concentration just high enough to elicit a maximum response. The exact concentration of antibody required to neutralize human NGFB activity is dependent on the cytokine concentration, cell type, growth conditions, and the type of activity studied. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanRat |
| Concentration | 0.5mg/ml |
| Purification | Protein A |
| Gene Synonyms | Beta-NGF HSAN5 MGC161426 MGC161428 NGFB Beta NGF BetaNGF |
|---|---|
| Target | NGF beta |
| Cellular Localization | Secreted |
| Application Notes | Dot: Use at a concentration of 1 - 3 μg/ml - determined using recombinant NGFB at 400 ng under non- and 200 ng under reducing conditions. ELISA: Use at a concentration of 1 - 2 μg/ml (100 μl/well) for capture ELISA, Monoclonal Anti-Human Nerve Growth Factor-Beta can be used as the capture antibody. Determined in combination with a biotinylated NGF-Beta polyclonal detection antibody at 50-100 ng/ml (100 μl/well), an ELISA for sample volumes of 100 μl with a range of 31.2 to 2000 pg/ml may be obtained. For capture ELISA, a working concentration of 1-2 μg/ml is determined to coat plates at 100 μl/well. Inhib: Use at a concentration of 1 - 2 μg/ml. Human NGFB stimulates the 3H-thymidine incorporation by TF-1 cells in a dose-dependent manner. The Neutralization Dose50 (ND50) for anti-human NGFB is approximately 1-3 μg/ml in the presence of 5 ng/ml of recombinant human NGFB using the NGFB responsive cell line TF-1. The ND50 is the concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when the cytokine is present at a concentration just high enough to elicit a maximum response. The exact concentration of antibody required to neutralize human NGFB activity is dependent on the cytokine concentration, cell type, growth conditions, and the type of activity studied. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: NoneConstituents: PBS |
