Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEUROG3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEUROG3.Application
Anti-NEUROG3 mouse monoclonal antibody (NEUROG3) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY NEUROG3Application
Immunohistochemical staining of paraffin-embedded liver tissue using anti-NEUROG3mouse monoclonal antibody. ( Dilution 1:50)| Product Name | NGN3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [3B5 ] to NGN3Involved in neurogenesis. Also required for the specification of a common precursor of the 4 pancreatic endocrine cell types. |
| Immunogen | Protein expressed in 293T cell transfected with human NGN3 expression vector |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 3B5 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIHC-PWB |
| Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NEUROG3 Atoh5 Math4B ngn3 |
|---|---|
| Target | NGN3 |
| Tissue Specificity | is specific for human NGN3. |
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEUROG3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEUROG3.
Application
Anti-NEUROG3 mouse monoclonal antibody (NEUROG3) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY NEUROG3
Application
Immunohistochemical staining of paraffin-embedded liver tissue using anti-NEUROG3mouse monoclonal antibody. ( Dilution 1:50)| Application Notes | Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
