Rabbit Polyclonal antibody to NIPP1This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq, Jul 2008]
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ARD1. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity
HumanMouseRatBovine
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
PRO2047 ARD1 protein phosphatase 1 regulatory subunit 8 PPP1R8
Target
NIPP1
Tissue Specificity
Recognizes endogenous levels of ARD1 protein.
Application
Application
Western blot analysis of ARD1 expression in HeLa (A); SP2/0 (B); H9C2 (C) whole cell lysates.
Application Notes
WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form
Liquid
Storage Instructions
Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles.
Storage Buffer
Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide.