| Product Name | Nitrotyrosine antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [39B6] to NitrotyrosineNitrotyrosine is a marker for inflammation and nitric oxide (NO) production and is formed in the presence of the active metabolite NO. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase, and prostacyclin synthase. Because nitrotyrosine is a stable product of multiple pathways, such as the formation of peroxynitrite, its plasma concentration may be a useful determinant of NO-dependent damage in vivo. Nitrotyrosine has been detected in inflammatory diseases such as septic shock, rheumatoid arthritis, celiac disease, atherosclerosis, and chronic renal failure. |
| Immunogen | 3-(4-hydroxy-3-nitrophenylacetamido) propionic acid conjugated to BSA |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 39B6 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-PIPWB |
| For WB: Use at a concentration of 0.2-2ug/ml For IHC: Use at a concentration of 1-5ug/ml. For IP: Use 1-2ug per 100-500ug total protein. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseDogRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 3 Nitrotyrosine 3Nitrotyrosine |
|---|---|
| Target | Nitrotyrosine |
| Tissue Specificity | This antibody recognizes 3-nitrotyrosine moieties in human, mouse, rat, and dog samples. |
| Positive Control | Rat brain tissue extract or nitrated BSA |
|---|---|
| Application Notes | For WB: Use at a concentration of 0.2-2ug/ml For IHC: Use at a concentration of 1-5ug/ml. For IP: Use 1-2ug per 100-500ug total protein. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS, 50% glycerol with 0.02% sodium azide as preservative |
