Application
Western blot analysis of NKX2-1 in rat lung tissue lysate with NKX2-1 antibody at (A) 1 and (B) 2 ug/mL.Application
Immunohistochemistry of NKX2-1 in human lung tissue with NKX2-1 antibody at 2.5 ug/mL.Application
Immunofluorescence of NKX2-1 in human lung tissue with NKX2-1 antibody at 20 ug/mL.| Product Name | NKX2.1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NKX2.1This gene encodes a protein initially identified as a thyroid-specific transcription factor. The encoded protein binds to the thyroglobulin promoter and regulates the expression of thyroid-specific genes but has also been shown to regulate the expression of genes involved in morphogenesis. Mutations and deletions in this gene are associated with benign hereditary chorea, choreoathetosis, congenital hypothyroidism, and neonatal respiratory distress, and may be associated with thyroid cancer. Multiple transcript variants encoding different isoforms have been found for this gene. This gene shares the symbol/alias 'TFF1' with another gene, transcription termination factor 1, which plays a role in ribosomal gene transcription. [provided by RefSeq, Apr 2011] |
| Immunogen | NKX2-1 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human NKX2-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| WB: 1 - 2 ug/ml. IHC-P: 2.5 ug/ml. IF: 20 ug/ml: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | TEBP TITF1 NK-2 TTF-1 BHC NK2 homeobox 1 BCH NKX2-1 TTF1 NKX2A |
|---|---|
| Target | NKX2.1 |
| Tissue Specificity | NKX2-1 antibody is predicted to not cross-react with other NK2 homeobox family members. At least three isoforms of NKX2-1 are known to exist; this antibody will detect all three. |
Application
Western blot analysis of NKX2-1 in rat lung tissue lysate with NKX2-1 antibody at (A) 1 and (B) 2 ug/mL.
Application
Immunohistochemistry of NKX2-1 in human lung tissue with NKX2-1 antibody at 2.5 ug/mL.
Application
Immunofluorescence of NKX2-1 in human lung tissue with NKX2-1 antibody at 20 ug/mL.| Application Notes | WB: 1 - 2 ug/ml. IHC-P: 2.5 ug/ml. IF: 20 ug/ml: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Be stored at 4oC for three months and -20oC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
