Application
Immunofluorescence staining of methanol-fixed Hela cells using NMDAR2B(phospho-Tyr1474) antibodyApplication
Western blot analysis of extracts from A431 cells untreated or treated with EGF using NMDAR2B (phospho-Tyr1474) Antibody| Product Name | NMDAR2B (phospho Tyr1474) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NMDAR2B (Phospho Tyr1474)NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. |
| Immunogen | Peptide sequence around phosphorylation site of Tyr1474 (H-V-Y(p)-E-K) derived from Human NMDAR2B. |
| Modification | p-Tyr1474 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Suggested starting dilutions are as follows:
For ICC/IF: 1:100-1:200.
For WB: 1:500-1:1000
Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC142178 MGC142180 NMDAR2B NR2B hNR3 GRIN2B glutamate receptor subunit epsilon-2 N-methyl-D-aspartate receptor subunit 3 glutamate [NMDA] receptor subunit epsilon-2 NR3 N-methyl D-aspartate receptor subtype 2B glutamate receptor ionotropic N- |
|---|---|
| Target | NMDAR2B (Phospho Tyr1474) |
| Molecular Weight(MW) | 190 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using NMDAR2B(phospho-Tyr1474) antibody
Application
Western blot analysis of extracts from A431 cells untreated or treated with EGF using NMDAR2B (phospho-Tyr1474) Antibody| Application Notes | Suggested starting dilutions are as follows:
For ICC/IF: 1:100-1:200.
For WB: 1:500-1:1000
Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
