Application
WB analysis of HepG2 lysate (35ug) using NMNAT1 antibody at a dilution of 1:1,000.| Product Name | NMNAT1 antibody [AT4A2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [AT4A2] to NMNAT1This gene encodes an enzyme which catalyzes a key step in the biosynthesis of the coenzyme NAD. The encoded protein is one of several nicotinamide nucleotide adenylyltransferases. Studies in Drosophila and mammalian neurons have shown the encoded protein can confer protection to damaged neurons. This protection requires enzymatic activity which increases NAD levels and activates a nuclear deacetylase which is the protective molecule. Pseudogenes of this gene are located on chromosomes 1, 3, 4, 14 and 15. [provided by RefSeq, Dec 2011] |
| Immunogen | Recombinant human NMNAT1 (1-279aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | AT4A2 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFWB |
| The antibody has been tested by ELISA and Western blot analyses to assure specificity and reactivity. Since applications vary, each investigation should be titrated by a reagent to obtain optimal results. Recommended dilution range for Western blot is 1:500 ~ 1000. Recommended starting dilution is 1:500 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PNAT1 , LCA9 , nicotinamide nucleotide adenylyltransferase 1 , NMNAT |
|---|---|
| Target | NMNAT1 |
| Light Chain Type | kappa |
Application
WB analysis of HepG2 lysate (35ug) using NMNAT1 antibody at a dilution of 1:1,000.| Application Notes | The antibody has been tested by ELISA and Western blot analyses to assure specificity and reactivity. Since applications vary, each investigation should be titrated by a reagent to obtain optimal results. Recommended dilution range for Western blot is 1:500 ~ 1000. Recommended starting dilution is 1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide |
