Application
Immunohistochemistry was performed on normal deparaffinized human Kidney tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:500 with or without nmt55 / p54nrb antibody [78-1 C] overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.| Product Name | nmt55 / p54nrb antibody [78-1 C] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [78-1 C] to nmt55 / p54nrbThis gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq, Feb 2009] |
| Immunogen | Synthetic peptide corresponding to residues T(140) V R E A G P P A F Y R P N S(154) of human ER alpha. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 78-1 C |
| Host Species | Mouse |
| Tested Applications | IHCIHC-PIPWB |
| IHC: 1:200 | |
| Species Reactivity | HumanRatRabbitBovine |
| Concentration | 1mg/ml |
| Gene Synonyms | P54 , non-POU domain containing, octamer-binding , NONO , NRB54 , P54NRB , NMT55 |
|---|---|
| Target | nmt55/p54nrb |
Application
Immunohistochemistry was performed on normal deparaffinized human Kidney tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:500 with or without nmt55 / p54nrb antibody [78-1 C] overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.| Application Notes | IHC: 1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20° C, Avoid Freeze/Thaw Cycles |
| Storage Buffer | Ascites containing 0.05 % sodium azide |
