Application
Western Blot: n-Myc Antibody (NMYC-1) - Western blot analysis of n-Myc expression in human heart tissue.Application
Western Blot: n-Myc Antibody (NMYC-1) - Western blot analysis of n-Myc expression in IMR32 whole cell lysate.Application
Immunofluorescence: n-Myc Antibody (NMYC-1) - The n-Myc (NMYC-1) antibody was tested in NTERA2 cells at a 1:50 dilution against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.| Product Name | n-Myc antibody |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Mouse monoclonal [NMYC-1] to n-MycMembers of the Myc gene (c-Myc, n-Myc, and L-Myc) family code for proteins that homodimerize but do not bind well to DNA. Myc-Max heterodimers, however, bind with great affinity to the sequence CACGTG and act to initiate transcription of target genes. |
| Immunogen | Synthetic peptide: SPYVESEDAPPQKC conjugated to KLH, corresponding to amino acids 327-339 of Human n-Myc. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | NMYC-1 |
| Host Species | Mouse |
| Tested Applications | GSAICC/IFIPWBChIP |
| Recommended Starting Dilutions:
For WB: Use at a dilution of 1:500-1:1000.
For ChIP: Use at a dilution of 1:10-1:500.
For GS: Use at a dilution of 1:1-1:100.
For ICC/IF: Use at a dilution of 1:50-1:100.
For IP: Use at a dilution of 1:10-1:100.
Optimal dilutions should be determined experimentally by the researcher.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | P04198 nMyc ODED MYCN 164280 MODED 4613 N-myc n Myc |
|---|---|
| Target | n-Myc |
| Tissue Specificity | This antibody does not react with c-Myc or L-Myc. |
| Light Chain Type | kappa |
Application
Western Blot: n-Myc Antibody (NMYC-1) - Western blot analysis of n-Myc expression in human heart tissue.
Application
Western Blot: n-Myc Antibody (NMYC-1) - Western blot analysis of n-Myc expression in IMR32 whole cell lysate.
Application
Immunofluorescence: n-Myc Antibody (NMYC-1) - The n-Myc (NMYC-1) antibody was tested in NTERA2 cells at a 1:50 dilution against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.| Application Notes | Recommended Starting Dilutions:
For WB: Use at a dilution of 1:500-1:1000.
For ChIP: Use at a dilution of 1:10-1:500.
For GS: Use at a dilution of 1:1-1:100.
For ICC/IF: Use at a dilution of 1:50-1:100.
For IP: Use at a dilution of 1:10-1:100.
Optimal dilutions should be determined experimentally by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Tris-glycine, 150 mM NaCl and 0.05% Sodium Azide as preservative. |
