Application
Western blots of whole yeast protein extracts with a collection of our antibodies. The blot for is in the indicated lane, and the number indicates the SDS-PAGE molecular weight in kDa.| Product Name | Nop1p antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [28F2] to Nop1p - Nucleolar MarkerNop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is fibrillarin (accession P22087) a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human fibrillarin gene is located on chromosome 19 (19q13.1). Fibrillarin proteins have been cloned and sequenced from several other species (Mouse, accession P35550, Xenopus accession P22232, C. elegans accession Q22053, and S. pombe accession P35551). The N terminal ~80 amino acids contain multiple copies based on the peptide RGG and the remaining ~240 amino acids consist of the fibrillarin domain. A fibrillarin homologue has also been identified in the genome of the archean Methanococcus (accession NC_000909). This protein lacks the RGG rich N-terminal extension but is clearly homologues to the other sequences throughout all of the fibrillarin domain. The structure of this molecule has been determined and shown to consist of 2 extended b-sheets flanked by 4 alpha helixes. Patients with the autoimmune disease scleroderma often have circulating auto-antibodies to a 34 kDa protein which was subsequently found to be fibrillarin. |
| Immunogen | Nuclear preparation (S. cerevisiae).Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG3 |
| Clone Number | 28F2 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| IF: Use at a dilution of 1/2000 - 1/20000 (yeast cells). IP: Use 2-4 μl for an amount of lysate derived from 5 OD600 units of yeast culture. WB: Use at a dilution of 1/10000 (cell lysates)- 1/25000 (nuclear fractions)(yeast protein samples). Predicted molecular weight: 34 kDa. For other (non-ECL) western detection methods, use at a dilution of 1/1000 - 1/5000. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | Yeast |
| Concentration | 1mg/ml |
| Gene Synonyms | LOT3 |
|---|---|
| Target | Nop1p |
| Cellular Localization | Nuclear (nucleolus) |
Application
Western blots of whole yeast protein extracts with a collection of our antibodies. The blot for is in the indicated lane, and the number indicates the SDS-PAGE molecular weight in kDa.| Application Notes | IF: Use at a dilution of 1/2000 - 1/20000 (yeast cells). IP: Use 2-4 μl for an amount of lysate derived from 5 OD600 units of yeast culture. WB: Use at a dilution of 1/10000 (cell lysates)- 1/25000 (nuclear fractions)(yeast protein samples). Predicted molecular weight: 34 kDa. For other (non-ECL) western detection methods, use at a dilution of 1/1000 - 1/5000. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 10mM Sodium Azide; Constituents: Tissue Culture Supernatant |
