| Product Name | Nop2p antibody [22G1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [22G1] to Nop2p - Nucleolar Marker |
| Immunogen | This was raised against a yeast nucleolar prep, and screened by immunocytochemistry and western blotting. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG3 |
| Clone Number | 22G1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| WB: Use at a dilution of 1/10000 (cell lysates) - 1/25000 (nuclear fractions). For other (non-ECL) detection methods, use at a dilution of 1/1000 - 1/5000. Predicted molecular weight: 100 kDa. IF: Use at a dilution of 1/500 - 1/5000 (yeast cells). Optimal dilutions/concentrations should be determined by the end user. | |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Target | Nop2p |
|---|---|
| Tissue Specificity | Specificity was determined by western blot analysis of extracts from a yeast strain expressing Nop2p under GAL-UAS control and from strains containing nop2 ts alleles that express Nop2p isoforms of altered molecular weight. |
| Cellular Localization | Nuclear (nucleolus) |
| Light Chain Type | kappa |
| Application Notes | WB: Use at a dilution of 1/10000 (cell lysates) - 1/25000 (nuclear fractions). For other (non-ECL) detection methods, use at a dilution of 1/1000 - 1/5000. Predicted molecular weight: 100 kDa. IF: Use at a dilution of 1/500 - 1/5000 (yeast cells). Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles. |
| Storage Buffer | Preservative: 10mM Sodium Azide; Constituents: Tissue Culture Supernatant |
