| Product Name | NOTCH1 antibody [A6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [A6] to Notch1The notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine rich EGF like repeats in the extracellular region. Four notch genes have been described in mammals, which include notch 1. notch 2, notch 3, and notch 4(Int 3), which have been implicated in the differentiation of the nervous system and other structures. The EGF like proteins Delta and Serrate have been identified as ligands of notch 1. Mature notch proteins are heterodimeric receptors derived from the cleavage of notch preproteins into an extracellular subunit (NEC) containing multiple EGF like repeats and a transmembrane subunit including intracellular region (Ntm). Alteration of notch 1 signaling or expression may contribute to tumorigenesis. |
| Immunogen | Recombinant protein encoding the ligand binding region of human Notch 1 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | A6 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-P |
| For FACS: Use at an assay dependent dilution. For IHC-P: Use at 1/50 for 30 minute at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at room temperature for 20 min. Optimal dilutions / concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouse |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | TAN1, hN1 |
|---|---|
| Target | Notch1 |
| Tissue Specificity | This antibody has not been tested for its cross reactivity with Notch 2. |
| Application Notes | For FACS: Use at an assay dependent dilution. For IHC-P: Use at 1/50 for 30 minute at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at room temperature for 20 min. Optimal dilutions / concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH7.4, containing 0.2% BSA and 0.1% sodium azide |
