Application
Immunohistochemistry of NOTUM in human brain tissue with NOTUM antibody at 2.5 ug/ml.Application
Western blot analysis of NOTUM in human kidney tissue lysate with NOTUM antibody at (A) 0.5 and (B) 1 ug/ml.Application
Immunofluorescence of NOTUM in Mouse Kidney cells with NOTUM antibody at 20 ug/mL.| Product Name | NOTUM antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to NOTUMIn Drosophila, the notum gene is regulated by the Wingless pathway and encodes a secreted hydrolase that modifies heparan sulfate proteoglycans. The mammalian homolog has been shown to be able to cleave glypicans and can release GPI-anchored proteins from the mammalian cell surface. Like the Drosophilia NOTUM, the mammalian protein can act as a negative regulator of the Wnt signaling pathway. NOTUM is expressed at a low level in most mammalian tissues, although it is overexpressed in a subset of human hepatocellular carcinomas. Its transcription is regulated by b-catenin/TCF and is a target of the Wnt signaling pathway, forming a negative feedback loop that regulates the expression and activity of the Wnt pathway. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | NOTUM NOTUM pectinacetylesterase homolog |
|---|---|
| Target | NOTUM |
| Tissue Specificity | This antibody is specific for NOTUM |
Application
Immunohistochemistry of NOTUM in human brain tissue with NOTUM antibody at 2.5 ug/ml.
Application
Western blot analysis of NOTUM in human kidney tissue lysate with NOTUM antibody at (A) 0.5 and (B) 1 ug/ml.
Application
Immunofluorescence of NOTUM in Mouse Kidney cells with NOTUM antibody at 20 ug/mL.| Positive Control | Blocking Peptide: Cat.No. GTX85260-PEP - NOTUM Peptide Lysates: Cat. No. GTX27920 - Human Kidney Tissue Lysate |
|---|---|
| Application Notes | ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.02% sodium azide |
