Application
Immunohistochemistry of NOTUM in human brain tissue with NOTUM antibody at 2.5 μg/mL.Application
Immunofluorescence of NOTUM in Mouse Kidney cells with NOTUM antibody at 20 μg/mL.Application
Western blot analysis of NOTUM in human kidney tissue lysate with NOTUM antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | NOTUM Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NOTUM Antibody: Protein notum homolog |
| Product description | NOTUM Antibody: In Drosophila, the notum gene is regulated by the Wingless pathway and encodes a secreted hydrolase that modifies heparan sulfate proteoglycans. The mammalian homolog has been shown to be able to cleave glypicans and can release GPI-anchored proteins from the mammalian cell surface. Like the Drosophilia NOTUM, the mammalian protein can act as a negative regulator of the Wnt signaling pathway. NOTUM is expressed at a low level in most mammalian tissues, although it is overexpressed in a subset of human hepatocellular carcinomas. Its transcription is regulated by beta-catenin/TCF and is a target of the Wnt signaling pathway, forming a negative feedback loop that regulates the expression and activity of the Wnt pathway.1) Giraldez AJ, Copley RR, and Cohen SM. HSPG modification by the secreted enzyme Notum shapes the wingless morphogen gradient. Dev. Cell2002; 667-76. |
| Immunogen | NOTUM antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human NOTUM. The immunogen is located within amino acids 90 - 140 of NOTUM. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| NOTUM antibody can be used for detection of NOTUM by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NOTUM |
|---|---|
| Alternative Names | NOTUM Antibody: Protein notum homolog |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemistry of NOTUM in human brain tissue with NOTUM antibody at 2.5 μg/mL.
Application
Immunofluorescence of NOTUM in Mouse Kidney cells with NOTUM antibody at 20 μg/mL.
Application
Western blot analysis of NOTUM in human kidney tissue lysate with NOTUM antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1305 - Human Kidney Tissue Lysate |
|---|---|
| Application Notes | NOTUM antibody can be used for detection of NOTUM by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NOTUM antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NOTUM Antibody is supplied in PBS containing 0.02% sodium azide. |
