Application
FACS analysis of human peripheral blood granulocytes using NOX2 / gp91phox antibody [NL7].| Product Name | NOX2 / gp91phox antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [NL7] to NOX2/gp91phoxCytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008] |
| Immunogen | NOX2/gp91phox solubilised in octyl-beta-glucopyranoside. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | NL7 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHC-FWB |
| Flow Cytometry: 1/25 - 1/200 Optimal dilution / concentration should be determined by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CYBB , CGD , GP91-PHOX , GP91PHOX , AMCBX2 , P91-PHOX , GP91-1 |
|---|---|
| Target | NOX2/gp91phox |
| Tissue Specificity | This antibody recognizes the native as well as the denatured form of the protein. Clone NL7 has an inhibitory effect on NADPH oxidase activation. |
Application
FACS analysis of human peripheral blood granulocytes using NOX2 / gp91phox antibody [NL7].| Application Notes | Flow Cytometry: 1/25 - 1/200 Optimal dilution / concentration should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | Phosphate buffered saline containing 0.09% Sodium Azide (NaN3) |
