Application
Bouin-fixed, paraffin-embedded Chronic Lymphocytic Leukemia (CLL) xenograft stained with Noxa antibody (1:2000), peroxidase-conjugate and DAB chromogen. Staining for 2 hr at RT was used.Application
Western blot detection of Noxa antibody in A) 293 cells transfected with human Noxa, B) RL-7 cell (a follicular lymphoma) and C) T98G lysate using at 2 µg/ml.| Product Name | Noxa antibody [114C307] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [114C307] to NoxaRecently a BH3 only member of the Bcl2 family has been identified in human and mouse and designated as Noxa (for damage). The expression of the Noxa gene involves direct activation of its promoter by p53. Increased expression of Noxa protein occurs in normal thymocytes but not in p53 deficient thymocytes. Noxa cDNA codes for a 103 amino acid protein. The co-immunoprecipitation data suggest that Noxa protein may interact with proteins belonging to the Bcl2 family, such as, BclXL and Mcl1. It has also been shown that blocking the endogenous Noxa induction results in the suppression of apoptosis. Treatment of cells with Noxa antisense oligonucleotides blocks radiation induced apoptosis suggesting that Noxa may represent a mediator of p53 dependent apoptosis. |
| Immunogen | Fusion protein containing human Noxa. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 114C307 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| Recommended Starting Dilutions: For WB: Use at a concentration of 1-2 ug/ml. For IHC-P: Use at a concentration of 5 ug/ml. For ICC/IF: Use at a dilution of 1:10-1:500. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouse |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | APR, NOXA |
|---|---|
| Target | Noxa |
| Cellular Localization | Mitochondrion, cytosol, nucleus |
Application
Bouin-fixed, paraffin-embedded Chronic Lymphocytic Leukemia (CLL) xenograft stained with Noxa antibody (1:2000), peroxidase-conjugate and DAB chromogen. Staining for 2 hr at RT was used.
Application
Western blot detection of Noxa antibody in A) 293 cells transfected with human Noxa, B) RL-7 cell (a follicular lymphoma) and C) T98G lysate using at 2 µg/ml.| Application Notes | Recommended Starting Dilutions: For WB: Use at a concentration of 1-2 ug/ml. For IHC-P: Use at a concentration of 5 ug/ml. For ICC/IF: Use at a dilution of 1:10-1:500. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS contains 0.05% BSA, 0.05% sodium azide |
