Application
Human Kidney (formalin-fixed, paraffin-embedded) stained with NPHS1 antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.| Product Name | NPHS1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to NPHS1This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes. |
| Immunogen | Synthetic peptide |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB (1 µg/ml), IHC-P (5 µg/ml): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CNF NPHN nephrin |
|---|---|
| Tissue Specificity | 14 amino acid peptide from near the carboxy terminus of human Nephrin. |
Application
Human Kidney (formalin-fixed, paraffin-embedded) stained with NPHS1 antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.| Application Notes | WB (1 µg/ml), IHC-P (5 µg/ml): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C, stable for one year. |
| Storage Buffer | PBS, 0.02% sodium azide. |
