Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using NQO1 antibody at 1:100 dilution.Application
NQO1 antibody detects NQO1 protein by Western blot analysis. A. 50 µg mouse heart lysate/extract 12 % SDS-PAGE NQO1 antibody dilution: 1:500Application
Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 12% SDS PAGE diluted at 1:1000| Product Name | NQO1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NQO1 (NAD(P)H dehydrogenase, quinone 1)This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NQO1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Rhesus Monkey, Bovine, Guinea pigRat (83%), Pig (92%), Rhesus Monkey (98%), Bovine (92%), Guinea pig (86%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DHQU antibody DIA4 antibody DTD antibody NMOR1 antibody NMORI antibody QR1 antibody NQO1 antibody phylloquinone reductase antibody dioxin-inducible 1 antibody azoreductase antibody NAD(P)H:Quinone acceptor oxidoreductase type 1 antibody quinone |
|---|---|
| Molecular Weight(MW) | 31 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using NQO1 antibody at 1:100 dilution.
Application
NQO1 antibody detects NQO1 protein by Western blot analysis. A. 50 µg mouse heart lysate/extract 12 % SDS-PAGE NQO1 antibody dilution: 1:500
Application
Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 12% SDS PAGE diluted at 1:1000| Positive Control | HeLa , HepG2 , mouse heart |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
