Application
Sample (30 µg of whole cell lysate) A: Molt-4 B: Raji 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
NQO1 antibody [C2C3], C-term detects NQO1 protein at cytoplasm in human rectal carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human rectal carcinoma. NQO1 antibody [C2C3], C-term diluted at 1:500.Application
NQO1 antibody [C2C3], C-term detects NQO1 protein by western blot analysis. A. 50 µg mouse heart lysate/extract 12% SDS-PAGE NQO1 antibody [C2C3], C-term dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
NQO1 antibody [C2C3], C-term detects NQO1 protein by western blot analysis. A. 50 µg rat heart lysate/extract 12% SDS-PAGE NQO1 antibody [C2C3], C-term dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NQO1 antibody at 1:200 dilution.| Product Name | NQO1 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NQO1 (NAD(P)H dehydrogenase, quinone 1)This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NQO1. The exact sequence is proprietary. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB:1:500~1:2000 ICC/IF:1:100~1:500 IHC-P:1:50~1:200 |
|
| Species Reactivity | HumanMonkeyMouseRat |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DHQU antibody DIA4 antibody DTD antibody NMOR1 antibody NMORI antibody QR1 antibody NQO1 antibody phylloquinone reductase antibody dioxin-inducible 1 antibody azoreductase antibody NAD(P)H:Quinone acceptor oxidoreductase type 1 antibody quinone |
|---|---|
| Molecular Weight(MW) | 31 kDa (note) (4) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 µg of whole cell lysate) A: Molt-4 B: Raji 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
NQO1 antibody [C2C3], C-term detects NQO1 protein at cytoplasm in human rectal carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human rectal carcinoma. NQO1 antibody [C2C3], C-term diluted at 1:500.
Application
NQO1 antibody [C2C3], C-term detects NQO1 protein by western blot analysis. A. 50 µg mouse heart lysate/extract 12% SDS-PAGE NQO1 antibody [C2C3], C-term dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
NQO1 antibody [C2C3], C-term detects NQO1 protein by western blot analysis. A. 50 µg rat heart lysate/extract 12% SDS-PAGE NQO1 antibody [C2C3], C-term dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NQO1 antibody at 1:200 dilution.| Positive Control | Molt-4 , Raji , mouse heart , rat heart |
|---|---|
| Application Notes | WB:1:500~1:2000 ICC/IF:1:100~1:500 IHC-P:1:50~1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
