Application
Immunocytochemistry/Immunofluorescence: NQO1 Antibody (A180) [ - NQO1 Antibody (A180) [ - NQO1 antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).Application
Immunohistochemistry: NQO1 Antibody (A180) [ - Analysis of NQO1 in human breast cancer using DAB with hematoxylin counterstain.Application
Western Blot: NQO1 Antibody (A180) [ - Detection of NQO1 in A549 lysates using Lane 1: 0.5 ug/ml. Lane 2: 2 ug/ml. Exposure: 1 second.Application
Application| Product Name | NQO1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody to NQO1NQO1 is a 2-electron reductase that detoxifies quinones derived from the oxidation of phenolic metabolites of benzene. Individuals homozygous for the T/T form of the 609C-T polymorphism have an increased risk of benzene hematotoxicity. |
| Immunogen | Full length recombinant NQO1 (from human lung). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | A180 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-FIHC-PIPWB |
| This antibody works well in Western blot where a single band at ~31 kDa is seen. No other applications have been tested. Past productions of this antibody have been used for immunoprecipitation, immunofluorescence, and immunohistochemistry (in human, monkey, and canine formalin-fixed paraffin-embedded sections, frozen sections not tested).Suggested working dilutions: *Western blot: 1:1,000-1:10,000 (ECL)Immunohistochemistry: (+)Immunofluorescence: (+) Immunoprecipitation: (+)*The investigator should determine the optimal working dilution for a specific application.: |
|
| Species Reactivity | HumanMouseDogMonkeyRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | DHQU DIA4 DTD NMOR1 NMORI QR1 |
|---|---|
| Target | NQO1 |
| Tissue Specificity | This antibody is specific for NQO1. It does not cross-react with NQO2. |
| Cellular Localization | Cytoplasmic (primarily), nuclear (faintly) |
Application
Immunocytochemistry/Immunofluorescence: NQO1 Antibody (A180) [ - NQO1 Antibody (A180) [ - NQO1 antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Application
Immunohistochemistry: NQO1 Antibody (A180) [ - Analysis of NQO1 in human breast cancer using DAB with hematoxylin counterstain.
Application
Western Blot: NQO1 Antibody (A180) [ - Detection of NQO1 in A549 lysates using Lane 1: 0.5 ug/ml. Lane 2: 2 ug/ml. Exposure: 1 second.
Application
Application| Application Notes | This antibody works well in Western blot where a single band at ~31 kDa is seen. No other applications have been tested. Past productions of this antibody have been used for immunoprecipitation, immunofluorescence, and immunohistochemistry (in human, monkey, and canine formalin-fixed paraffin-embedded sections, frozen sections not tested).Suggested working dilutions: *Western blot: 1:1,000-1:10,000 (ECL)Immunohistochemistry: (+)Immunofluorescence: (+) Immunoprecipitation: (+)*The investigator should determine the optimal working dilution for a specific application.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot into working amounts and store at -20C. Avoid freeze/thaw cylces. |
| Storage Buffer | Tris-glycine and 150mM NaCl containing 0.05% sodium azide. |
