Mouse monoclonal antibody [AT1E3] to NQO2NQO2 (EC 1.10.99.2) is a flavoprotein that catalyzes the 2-electron reduction of various quinones, redox dyes, and the vitamin K menadione. NQO2 predominantly uses dihydronicotinamide riboside (NRH) as the electron donor (summary by Wu et al., 1997 [PubMed 9367528]).[supplied by OMIM, Jul 2010]
Immunogen
Recombinant human NQO2 (1-231aa) purified from E. coli
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
AT1E3
Host Species
Mouse
Tested Applications
ELISAWB
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:250 ~ 500. Recommended starting dilution is 1:500:
Species Reactivity
HumanMouse
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
WB analysis of mouse liver lysate (35ug) using NQO2 antibody at a dilution of 1:500.
Application Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:250 ~ 500. Recommended starting dilution is 1:500:
Additional Information
Form
Liquid
Storage Instructions
Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
Storage Buffer
Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide