Application
HEK293T cells transfected with either NRBP1 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NRBP1 antibody , and then analyzed by flow cytometry.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NRBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NRBP1.Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-anti-NRBP1monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-NRBP1mouse monoclonal antibody. ( Dilution 1:50)| Product Name | NRBP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [7E2] to NRBP1May play a role in subcellular trafficking between the endoplasmic reticulum and Golgi apparatus through interactions with the Rho-type GTPases. Binding to the NS3 protein of dengue virus type 2 appears to subvert this activity into the alteration of the intracellular membrane structure associated with flaviviral replication. |
| Immunogen | Protein expressed in 293T cell transfected with human NRBP1 expression vector |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 7E2 |
| Host Species | Mouse |
| Tested Applications | FACSIHCIHC-PWB |
| Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanDogMonkeyRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NRBP NRBP1 MUDPNP BCON3 MADM |
|---|---|
| Target | NRBP1 |
| Tissue Specificity | is specific for human NRBP1. |
| Cellular Localization | Colocalizes with activated RAC3 to endomembranes and at the cell periphery in lamellipodia. |
Application
HEK293T cells transfected with either NRBP1 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NRBP1 antibody , and then analyzed by flow cytometry.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NRBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NRBP1.
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-anti-NRBP1monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-NRBP1mouse monoclonal antibody. ( Dilution 1:50)| Application Notes | Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
