Application
Cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human NSD3 (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Product Name | NSD3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2E9 ] to NSD3This gene encoding NSD3 is related to the Wolf-Hirschhorn syndrome candidate - 1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. NSD3 is Histone methyltransferase which preferentially methylates 'Lys-4' and 'Lys-27' of histone H3 and is amplified in several tumor cell lines and primary breast carcinomas. |
| Immunogen | Recombinant human NSD3 (383-660aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 2E9 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western Blot: Use at 1:1,000 ~ 3,000.Optimal working concentrations should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | NSD 3 pp14328 WHSC1L1 protein DKFZp667H044 Nuclear SET domain containing protein3 Whistle WHSC1L1 WHSC1 like protein1 Wolf Hirschhorn syndrome candidate 1 like 1 WHSC1 like protein 1 NSD3 Transcr FLJ20353 Wolf Hirschhorn syndrome candidate1 l |
|---|---|
| Target | NSD3 |
| Light Chain Type | kappa |
Application
Cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human NSD3 (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Application Notes | Western Blot: Use at 1:1,000 ~ 3,000.Optimal working concentrations should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
