Application
NSE antibody [C1C3] detects NSE protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NSE protein stained by NSE antibody [C1C3] diluted at 1:500. Blue: Hoechst 33342 staining.Application
NSE antibody [C1C3] detects NSE protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: NSE protein stained by NSE antibody [C1C3] diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .Application
Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:10000Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 10% SDS PAGE diluted at 1:1000| Product Name | NSE antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NSEThis gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ENO2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Chicken, Rhesus Monkey, BovineZebrafish (89%), Chicken (96%), Rhesus Monkey (100%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | NSE antibody ENO2 antibody 2-phospho-D-glycerate hydro-lyase antibody neural enolase antibody neuron-specific enolase antibody gamma-enolase antibody 2-phospho-D-glycerate hydrolyase antibody neurone-specific enolase antibody neuron specific gamma |
|---|---|
| Target | NSE |
| Molecular Weight(MW) | 47 kDa (note) (2) |
| Cellular Localization | Cytoplasm (By similarity) , Cell membrane (By similarity) |
Application
NSE antibody [C1C3] detects NSE protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NSE protein stained by NSE antibody [C1C3] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
NSE antibody [C1C3] detects NSE protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: NSE protein stained by NSE antibody [C1C3] diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .
Application
Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:10000
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 10% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HepG2 , Molt-4 , Raji , mouse brain |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
