Application
NSE antibody [N1C1] detects NSE protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: NSE protein stained by NSE antibody [N1C1] diluted at 1:250. Blue: Fluoroshield with DAPI .Application
Sample (50 µg of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample(30 µg of whole cell lysate) A:A431 B:H1299 10% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using ENO2 antibody at 1:100 dilution.| Product Name | NSE antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NSEThis gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ENO2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Chicken, Rhesus Monkey, BovineRat (99%), Zebrafish (83%), Chicken (89%), Rhesus Monkey (100%), Bovine (98%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NSE antibody ENO2 antibody 2-phospho-D-glycerate hydro-lyase antibody neural enolase antibody neuron-specific enolase antibody gamma-enolase antibody 2-phospho-D-glycerate hydrolyase antibody neurone-specific enolase antibody neuron specific gamma |
|---|---|
| Target | NSE |
| Molecular Weight(MW) | 47 kDa (note) |
| Cellular Localization | Cytoplasm (By similarity) , Cell membrane (By similarity) |
Application
NSE antibody [N1C1] detects NSE protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: NSE protein stained by NSE antibody [N1C1] diluted at 1:250. Blue: Fluoroshield with DAPI .
Application
Sample (50 µg of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample(30 µg of whole cell lysate) A:A431 B:H1299 10% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using ENO2 antibody at 1:100 dilution.| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
