| Product Name | NSF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to NSFN ethylmaleimide sensitive factor (NSF) is an ~83 kDa protein (predicted MW) which was initially isolated based on its ability to restore intercisternal Golgi transport to N ethylmaleimide treated membranes. NSF was subsequently discovered to be involved in a variety of membrane fusion events. NSF is an ATPase with two ATP binding domains that are essential for membrane fusion and homo oligomerization. In order to interact with target membranes, NSF requires another set of soluble proteins called Soluble NSF attachment proteins (SNAPs). NSF has been shown to play a prominent role in synaptic vesicle fusion. Together with synaptotagmin and a SNAP, NSF modulates the interaction of SNAP25, VAMP, and syntaxin, in an ATP dependent manner, to form a complex with a sedimentation coefficient of 20 S. This complex may represent an intermediate involved in synaptic vesicle docking and fusion. |
| Immunogen | Synthetic peptide corresponding to amino acids around 731 of rat NSF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-FIPWB |
| IHC-Fr: Use at a concentration of 10 - 20 μg/ml. IP: Use at a concentration of 5 - 15 μg/ml. WB: Use at a concentration of 1 - 2 μg/ml. Predicted molecular weight: 83 kDa. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | MouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Nsf 60355 |
|---|---|
| Target | NSF |
| Cellular Localization | Cytoplasmic |
| Application Notes | IHC-Fr: Use at a concentration of 10 - 20 μg/ml. IP: Use at a concentration of 5 - 15 μg/ml. WB: Use at a concentration of 1 - 2 μg/ml. Predicted molecular weight: 83 kDa. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide. Constituents: 50% Glycerol, 1% BSA, PBS. |
