Application
Western blot of rat caudate lysate showing specific labeling of the ~75k NSF protein.| Product Name | NSF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Erg1, N-ethylmaleimide-sensitive fusion protein, NEM-sensitive fusion protein |
| Product description | NSF (N-ethylmaleimide sensitive fusion protein) is a critical component of the SNARE (soluble NSF attachment protein receptors) protein complex that is involved in synaptic vesicle trafficking. Specifically, NSF has been found to be essential in membrane fusion. Furthermore, NSF has been recently demonstrated to bind other protein complexes such as AMPA receptor subunits (GluR2), GATE-16, LMA-1 and Rabs suggesting a more diverse role in the assembly of various protein complexes (Whiteheart et al., 2004).1) Schweizer FE, Dresbach T, DeBello WM, O’Connor V, Augustine G, Betz H (1998) Regulation of neurotransmitter release kinetics by NSF. Science 279(5354):1203-1206. |
| Immunogen | Peptide corresponding to amino acid residues from the C-terminal region of rat NSF, conjugated to keyhole limpet hemocyanin (KLH). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| The antibody has been directly tested for reactivity in rat tissue. It is anticipated that the antibody will also work with human and mouse based on the fact that these species have 100% homology with the amino acid sequence used as antigen.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Nsf |
|---|---|
| Alternative Names | Erg1 N-ethylmaleimide-sensitive fusion protein NEM-sensitive fusion protein |
| Molecular Weight(MW) | 75 |
Application
Western blot of rat caudate lysate showing specific labeling of the ~75k NSF protein.| Application Notes | The antibody has been directly tested for reactivity in rat tissue. It is anticipated that the antibody will also work with human and mouse based on the fact that these species have 100% homology with the amino acid sequence used as antigen.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | NSF antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
