Application
Immunocytochemistry/Immunofluorescence: NSP 5 alpha 3 alpha Antibody [ - The NSP 5 alpha 3 antibody was tested in Hela cells at a 1:250 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained with Dylight 550 (Red) and DAPI (Blue), respectively.Application
Western Blot: NSP 5 alpha 3 alpha Antibody [ - Detection of NSP 5 alpha 3 alpha in HeLa whole cell lysate (RIPA) using 0.5ug/ml dilution, 15 second ECL detection| Product Name | NSP-5a3a antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to NSP 5a3aNSP type 5alpha/3alpha is a novel structure protein that may play a role in nuclear function during cell division. It is also highly expressed in certain tumor cell lines, suggesting that it may serve as a good tumor marker. |
| Immunogen | An internal synthetic peptide made to the human NSP5a3a protein sequence (between residues 1-100). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| This antibody is useful for Western blot analysis, where a band is seen ~80 kDa. A second band may be seen ~60 kDa. The specificity of this band is not known. No other applications have been tested. Western Blot: 1:500-1:2,000 (ECL)*Optimal working dilutions should be determined by the investigator.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | CYTSB FLJ36955 HCMOGT-1 NSP HCMOGT 1 NSP 5a3a HCMOGT1 |
|---|---|
| Target | NSP-5a3a |
| Tissue Specificity | This antibody is specific for NSP5a3a. |
Application
Immunocytochemistry/Immunofluorescence: NSP 5 alpha 3 alpha Antibody [ - The NSP 5 alpha 3 antibody was tested in Hela cells at a 1:250 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained with Dylight 550 (Red) and DAPI (Blue), respectively.
Application
Western Blot: NSP 5 alpha 3 alpha Antibody [ - Detection of NSP 5 alpha 3 alpha in HeLa whole cell lysate (RIPA) using 0.5ug/ml dilution, 15 second ECL detection| Positive Control | HeLa whole cell lysate |
|---|---|
| Application Notes | This antibody is useful for Western blot analysis, where a band is seen ~80 kDa. A second band may be seen ~60 kDa. The specificity of this band is not known. No other applications have been tested. Western Blot: 1:500-1:2,000 (ECL)*Optimal working dilutions should be determined by the investigator.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4 degrees Celcius. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | 0.1 ml of purified antibody in Tris-citrate/phosphate [pH 7-8] containing 0.1% sodium azide. |
