| Product Name | Nsr1p antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [31C4] to Nsr1p - Nucleolar MarkerNsr1p is localized in the nucleolus and binds to the single-stranded telomeric repeat sequence (TG1-3)n. It has a role in ribosomal RNA maturation and possibly in transport of proteins into the nucleus. |
| Immunogen | This was raised against a yeast nucleolar prep, and screened by immunocytochemistry and western blotting. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | 31C4 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| IF: Use at a dilution of 1/500 - 1/5000 (yeast cells). WB: Use at a dilution of 1/10000 (cell and nuclear lysates). For non-ECL western detection methods, use at a dilution of 1/1000 - 1/5000. Predicted molecular weight: 45 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Concentration | 1mg/ml |
| Target | Nsr1p |
|---|---|
| Tissue Specificity | This antibody was isolated during a screen for mAbs against yeast nucleolar proteins. Specificity was determined by screening a yeast genomic library in lambda gt11. Two individually isolated, different positive clones were found. Sequencing revealed that in both clones beta-galactosidase was fused at the EcoRI site to the amino acid sequence beginning with ?EFEH190 in Nsr1p. Thus, it is likely to react with an epitope(s) in the C-terminal 55% of Nsr1p. |
| Cellular Localization | Nuclear (nucleolus). |
| Application Notes | IF: Use at a dilution of 1/500 - 1/5000 (yeast cells). WB: Use at a dilution of 1/10000 (cell and nuclear lysates). For non-ECL western detection methods, use at a dilution of 1/1000 - 1/5000. Predicted molecular weight: 45 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 10mM Sodium Azide; Constituents: Tissue Culture Supernatant |
