| Product Name | NTF2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [4F5] to NTF2NTF2 (nuclear transport factor 2, also known as p10) an evolutionarily conserved protein that is essential for growth in yeast, plays an important role in nuclear protein import and Ran regulation. The properties of NTF2 suggest it may modulate the steady-state distribution of Ran. Both are imported into the nucleus as stoichiometric components of the import complex, and are required for efficient import. NTF2 regulates Ran distribution in living cells, and NTF2-mediated Ran nuclear import is required for NLS-dependent protein import in vitro. NTF2 binds directly to RanGDP but not RanGTP. In addition to binding RanGDP, NTF2 also binds NPC proteins. Mutations in NTF2 that abolish Ran binding do not affect binding to NPC proteins, which suggests that Ran and NPC proteins bind to different domains of NTF2. NTF2 binds directly to p62, as well as to other NPC proteins containing multiple FxFG peptide repeats. These repeat-containing NPC proteins have been proposed to provide binding sites for import complexes during translocation through the NPC. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 4F5 |
| Host Species | Mouse |
| Tested Applications | BlockingELISAICC/IFIPWB |
| BL: Use at an assay dependent dilution. ELISA: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 2 μg/ml. Detects a band of approximately 14 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseMonkeyRatHamsterXenopus |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | NTF2 PP15 |
|---|---|
| Target | NTF2 |
| Cellular Localization | Cytoplasmic |
| Positive Control | HeLa cytosolic extract. |
|---|---|
| Application Notes | BL: Use at an assay dependent dilution. ELISA: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 2 μg/ml. Detects a band of approximately 14 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 0.01M PBS. pH 7.4 |
