Application
Western Blot analysis using Nucleolin [4E2] antibody on HL60 (1) and ZR-75 (2) cell extractsApplication
Immunohistochemistry analysis of nuclear staining of colon cancer sections using Nucleolin mAb (4E2).| Product Name | Nucleolin antibody [4E2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [4E2] to NucleolinNucleolin, which is identical to human DNA helicase IV, is a major nucleolar phosphoprotein which is associated with preribosomal RNA and is implicated in the early stage of preribosomal RNP assembly and processing. This 100 kDa protein has three major domains: a N-terminal domain comprised of long acidic stretches interspersed with basic repeats, similar to the structure of a high mobility group-type protein (this domain is responsible for the ablility of nucleolin to modulate chromatin condensation), a central domain that contains four RNA binding elements, a C-terminal domain approximately 85 amino acids long that is rich in glycine, arginine, and phenylalanine residues. Nucleolin fluctuates in parallel to DNA synthesis; intact 100 kDa protein is the major species in actively dividing cells, whereas the degraded forms are relativley abundant in nondividing cells. Nucleolin can unwind RNA-RNA duplexes, as well as DNA-DNA and DNA-RNA duplexes. Nucleolin also interacts directly with DNA topoisomerase I. It is located mainly in dense fibrillar regions of the nucleolus. Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and preribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. It interacts with APTX and contains 4 RNA recognition motif (RRM) domains. |
| Immunogen | Human nucleolin protein from Raji cell extract. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4E2 |
| Host Species | Mouse |
| Tested Applications | ICC/IF,IHC,WB,ChIP |
| ICC: Use at a concentration of 1 - 10 μg/ml. IHC: Use at a concentration of 1 - 10 μg/ml. WB: Use at a concentration of 1 μg/ml. Predicted molecular weight: 100 kDa. This antibody allows in situ detection of nucleolar protein in Hep-II cell and paraffin embedded tissue section. Paraffin embedded sections should be processed under microwave treatment. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human,Bovine |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | C23, FLJ45706, FLJ59041 |
|---|---|
| Target | Nucleolin |
| Cellular Localization | Nuclear, located mainly in dense fibrillar regions of the nucleolus. |
Application
Western Blot analysis using Nucleolin [4E2] antibody on HL60 (1) and ZR-75 (2) cell extracts
Application
Immunohistochemistry analysis of nuclear staining of colon cancer sections using Nucleolin mAb (4E2).| Positive Control | Raji cell extract |
|---|---|
| Application Notes | ICC: Use at a concentration of 1 - 10 μg/ml. IHC: Use at a concentration of 1 - 10 μg/ml. WB: Use at a concentration of 1 μg/ml. Predicted molecular weight: 100 kDa. This antibody allows in situ detection of nucleolar protein in Hep-II cell and paraffin embedded tissue section. Paraffin embedded sections should be processed under microwave treatment. Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: NoneConstituents: 50% Glycerol, PBS |
