Application
Sample (30 µg of whole cell lysate) A: A431 B: H1299 10% SDS PAGE nucleoporin p62 antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Nucleoporin p62 antibody detects NUP62 protein by western blot analysis. A. 30 µg Neuro2A whole lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg BCL-1 whole cell lysate/extract F. 30 µg Raw264.7 whole cell lysate/extract G. 30 µg C2C12 whole cell lysate/extract 10% SDS-PAGE Nucleoporin p62 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Nucleoporin p62 antibody detects NUP62 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 10% SDS-PAGE Nucleoporin p62 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Nucleoporin p62 antibody detects Nucleoporin p62 protein at nuclear envelope on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. Nucleoporin p62 antibody diluted at 1:500.| Product Name | Nucleoporin p62 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Nucleoporin p62 (nucleoporin 62kDa)The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Nucleoporin p62. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Rhesus Monkey, BovineXenopus laevis (89%), Rhesus Monkey (99%), Bovine (96%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp547L134 antibody FLJ20822 antibody FLJ43869 antibody IBSN antibody MGC841 antibody SNDI antibody p62 antibody NUP62 antibody 62 kDa nucleoporin antibody nucleoporin Nup62 antibody nuclear pore glycoprotein p62 antibody nucleoporin 62kDa ant |
|---|---|
| Molecular Weight(MW) | 53 kDa (note) (5) |
| Cellular Localization | Nucleus , nuclear pore complex |
Application
Sample (30 µg of whole cell lysate) A: A431 B: H1299 10% SDS PAGE nucleoporin p62 antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Nucleoporin p62 antibody detects NUP62 protein by western blot analysis. A. 30 µg Neuro2A whole lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg BCL-1 whole cell lysate/extract F. 30 µg Raw264.7 whole cell lysate/extract G. 30 µg C2C12 whole cell lysate/extract 10% SDS-PAGE Nucleoporin p62 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Nucleoporin p62 antibody detects NUP62 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 10% SDS-PAGE Nucleoporin p62 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Nucleoporin p62 antibody detects Nucleoporin p62 protein at nuclear envelope on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. Nucleoporin p62 antibody diluted at 1:500.| Positive Control | A431 , H1299 , Neuro 2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
