Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 D: C2C12 7.5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: PC-12 7.5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using nucleoporin p62 antibody at 1:500 dilution.Application
Nucleoporin p62 antibody detects Nucleoporin p62 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and blotted with Nucleoporin p62 antibody diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Nucleoporin p62 antibody immunoprecipitates NUP62 protein in IP experiments. IP samples: 293T whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of NUP62 protein by 4 μg Nucleoporin p62 antibody 7.5 % SDS-PAGE The immunoprecipitated NUP62 protein was detected by Nucleoporin p62 antibody diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
Nucleoporin p62 antibody detects Nucleoporin p62 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nucleoporin p62 protein stained by Nucleoporin p62 antibody diluted at 1:750. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | Nucleoporin p62 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Nucleoporin p62 (nucleoporin 62kDa)The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Nucleoporin p62. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Rhesus Monkey, BovineRhesus Monkey (98%), Bovine (87%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp547L134 antibody FLJ20822 antibody FLJ43869 antibody IBSN antibody MGC841 antibody SNDI antibody p62 antibody NUP62 antibody 62 kDa nucleoporin antibody nucleoporin Nup62 antibody nuclear pore glycoprotein p62 antibody nucleoporin 62kDa ant |
|---|---|
| Molecular Weight(MW) | 53 kDa (note) (6) |
| Cellular Localization | Nucleus , nuclear pore complex |
Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 D: C2C12 7.5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: PC-12 7.5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using nucleoporin p62 antibody at 1:500 dilution.
Application
Nucleoporin p62 antibody detects Nucleoporin p62 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and blotted with Nucleoporin p62 antibody diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Nucleoporin p62 antibody immunoprecipitates NUP62 protein in IP experiments. IP samples: 293T whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of NUP62 protein by 4 μg Nucleoporin p62 antibody 7.5 % SDS-PAGE The immunoprecipitated NUP62 protein was detected by Nucleoporin p62 antibody diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
Nucleoporin p62 antibody detects Nucleoporin p62 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nucleoporin p62 protein stained by Nucleoporin p62 antibody diluted at 1:750. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | HepG2 , Molt-4 , Raji , NIH-3T3 , JC , BCL-1 , C2C12 , PC-12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
