Application
Nucleoporin p62 antibody [GT252] detects Nucleoporin p62 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract 7.5 % SDS-PAGE Nucleoporin p62 antibody [GT252] dilution: 1:1000Application
Nucleoporin p62 antibody [GT252] detects Nucleoporin p62 protein at nuclear envelope by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Nucleoporin p62 protein stained by Nucleoporin p62 antibody [GT252] diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | Nucleoporin p62 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT252] to Nucleoporin p62 (nucleoporin 62kDa)The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Nucleoporin p62. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | GT252 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus Monkey, BovineMouse (85%), Rat (84%), Rhesus Monkey (98%), Bovine (87%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | nucleoporin 62kDa p62 SNDI IBSN NUP62 |
|---|---|
| Target | Nucleoporin p62 |
| Cellular Localization | Nucleus , nuclear pore complex |
Application
Nucleoporin p62 antibody [GT252] detects Nucleoporin p62 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract 7.5 % SDS-PAGE Nucleoporin p62 antibody [GT252] dilution: 1:1000
Application
Nucleoporin p62 antibody [GT252] detects Nucleoporin p62 protein at nuclear envelope by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Nucleoporin p62 protein stained by Nucleoporin p62 antibody [GT252] diluted at 1:500. Blue: Hoechst 33342 staining.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
