Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with NuMA antibody [GT1510] diluted at 1:500.Application
NuMA antibody [GT1510] detects NuMA protein at centrosome by immunofluorescent analysis. Mitotic cells are highlighted in yellow box. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NuMA protein stained by NuMA antibody [GT1510] diluted at 1:500. Blue: Hoechst 33342 staining.Application
NuMA antibody [GT1510] detects NUMA1 protein by Western blot analysis. A. 30 µg A375 whole cell lysate/extract 5 % SDS-PAGE NuMA antibody [GT1510] dilution: 1:1000| Product Name | NuMA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT1510] to NuMA (nuclear mitotic apparatus protein 1) |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human NuMA. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GT1510 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (81%), Rat (83%), Bovine (89%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Molecular Weight(MW) | 238 kDa (note) (3) |
|---|---|
| Cellular Localization | Nucleus |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with NuMA antibody [GT1510] diluted at 1:500.
Application
NuMA antibody [GT1510] detects NuMA protein at centrosome by immunofluorescent analysis. Mitotic cells are highlighted in yellow box. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NuMA protein stained by NuMA antibody [GT1510] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
NuMA antibody [GT1510] detects NUMA1 protein by Western blot analysis. A. 30 µg A375 whole cell lysate/extract 5 % SDS-PAGE NuMA antibody [GT1510] dilution: 1:1000| Positive Control | 293T , A375 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
