Application
Immunohistochemical staining of paraffin-embedded human ovarian dysgerminoma using anti-Oct-4 RabMAb (cat. #).Application
Western blot analysis on (A) Seminoma (B) NCCIT and (C) HeLa cell lysates using anti-Oct-4 RabMAb (cat. # ).| Product Name | Oct4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EPR2054] to Oct-4Octamer transcription factor-4 (Oct-4) is a homeodomain transcription factor in the POU transcription factor family that specifically binds to the octamer motif (5'-ATTTCAT-3') . It is critical for early embryogenesis and for embryonic stem cell pluripotency, expressed mainly in the developing brain . OCT4 is a master regulator that affects the fate of pluripotent stem cells and germ cell precursors . For instance, the downregulation of Oct-4 triggers embryonic stem cell differentiation, simultaneous with gastrulation . It is also used as a marker for germ cell tumor diagnosis . |
| Immunogen | A synthetic peptide corresponding to residues in human Oct-4 was used as an immunogen. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EPR2054 |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PIPWB |
| For WB: Use at a dilution of 1:1,000 - 10,000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Oct-3 OTF4 OCT3 OCT4 Oct-4 MGC22487 OTF-3 OTF3 POU5F1 2011/10/3 2011/10/4 OTF 3 |
|---|---|
| Target | Oct-4 |
| Molecular Weight(MW) | 45 (note) |
Application
Immunohistochemical staining of paraffin-embedded human ovarian dysgerminoma using anti-Oct-4 RabMAb (cat. #).
Application
Western blot analysis on (A) Seminoma (B) NCCIT and (C) HeLa cell lysates using anti-Oct-4 RabMAb (cat. # ).| Application Notes | For WB: Use at a dilution of 1:1,000 - 10,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
