Application
Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of Oct4 by 2 μg of Oct4 antibody 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody diluted at 1:1000. EasyBlot anti-mouse IgG was used as a secondary reagent.Application
Sample (20 µg of whole cell lysate) A: mouse ESC 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: NT2D1 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Sample (10 µg of whole cell lysate) A: Non-transfected HeLa lysates B: HeLa transfected recombinant Oct4 protein 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Oct4 antibody [GT486] detects Oct4 protein by western blot analysis. A. 30 µg human ESC whole cell lysate/extract 10% SDS-PAGE dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Oct4 antibody detects Oct4 protein by immunofluorescent analysis. Sample: human embryonic stem cell were fixed in 4% paraformaldehyde for 15 min. Green: Oct4 protein stained by Oct4 antibody diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Oct4 antibody [GT486] detects POU5F protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Oct4 antibody [GT486] dilution: 1:50Application
Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using OCT3/4 antibody at 1:200 dilution.| Product Name | Oct4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT486] to OCT4 (POU class 5 homeobox 1)This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | GT486 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Cat, Pig, Rabbit, Rhesus Monkey, Chimpanzee, BovineCat (89%), Pig (88%), Rabbit (85%), Rhesus Monkey (97%), Chimpanzee (100%), Bovine (85%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC22487 antibody OTF-3 antibody OTF3 antibody OTF4 antibody POU5F1 antibody POU-type homeodomain-containing DNA-binding protein antibody octamer-binding protein 3 antibody POU domain transcription factor OCT4 antibody octamer-binding protein 4 ant |
|---|---|
| Molecular Weight(MW) | 39 kDa (note) |
| Cellular Localization | Nucleus |
Application
Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of Oct4 by 2 μg of Oct4 antibody 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody diluted at 1:1000. EasyBlot anti-mouse IgG was used as a secondary reagent.
Application
Sample (20 µg of whole cell lysate) A: mouse ESC 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: NT2D1 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Sample (10 µg of whole cell lysate) A: Non-transfected HeLa lysates B: HeLa transfected recombinant Oct4 protein 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Oct4 antibody [GT486] detects Oct4 protein by western blot analysis. A. 30 µg human ESC whole cell lysate/extract 10% SDS-PAGE dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Oct4 antibody detects Oct4 protein by immunofluorescent analysis. Sample: human embryonic stem cell were fixed in 4% paraformaldehyde for 15 min. Green: Oct4 protein stained by Oct4 antibody diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Oct4 antibody [GT486] detects POU5F protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Oct4 antibody [GT486] dilution: 1:50
Application
Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using OCT3/4 antibody at 1:200 dilution.| Positive Control | NT2D1 , human ESC , mouse ESC , HeLa transfected recombinant OCT3/4 protein |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
