Application
O-GlcNAc transferase antibody detects O-GlcNAc transferase protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: O-GlcNAc transferase protein stained by O-GlcNAc transferase antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.Application
O-GlcNAc transferase antibody detects O-GlcNAc transferase protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: O-GlcNAc transferase protein stained by O-GlcNAc transferase antibody diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Application
O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis. A. 30 µg PC-12 whole cell extract 5% SDS-PAGE O-GlcNAc transferase antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis. A. 30 µg 293T whole cell extract B. 30 µg A431 whole cell extract C. 30 µg HeLa whole cell extract D. 30 µg HepG2 whole cell extract 5% SDS-PAGE O-GlcNAc transferase antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 7.5% SDS-PAGE, and blotted with O-GlcNAc transferase antibody diluted by 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
O-GlcNAc transferase antibody detects O-GlcNAc transferase protein at cytoplasm and nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. O-GlcNAc transferase antibody diluted at 1:500.Application
Immunoprecipitation of O-GlcNAc transferase protein from A431 whole cell extracts using 5 μg of O-GlcNAc transferase antibody . Western blot analysis was performed using O-GlcNAc transferase antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | O-GlcNAc transferase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to O-GlcNAc transferase (O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase))O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human O-GlcNAc transferase. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Pig, BovineXenopus laevis (98%), Pig (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ23071 antibody HRNT1 antibody MGC22921 antibody O-GLCNAC antibody OGT antibody O-GlcNAc transferase p110 subunit antibody uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase antibody O-GlcNAc transferase subunit |
|---|---|
| Molecular Weight(MW) | 117 kDa (note) (2) |
| Cellular Localization | Cytoplasm (Potential) , Nucleus |
Application
O-GlcNAc transferase antibody detects O-GlcNAc transferase protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: O-GlcNAc transferase protein stained by O-GlcNAc transferase antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Application
O-GlcNAc transferase antibody detects O-GlcNAc transferase protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: O-GlcNAc transferase protein stained by O-GlcNAc transferase antibody diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Application
O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis. A. 30 µg PC-12 whole cell extract 5% SDS-PAGE O-GlcNAc transferase antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis. A. 30 µg 293T whole cell extract B. 30 µg A431 whole cell extract C. 30 µg HeLa whole cell extract D. 30 µg HepG2 whole cell extract 5% SDS-PAGE O-GlcNAc transferase antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 7.5% SDS-PAGE, and blotted with O-GlcNAc transferase antibody diluted by 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
O-GlcNAc transferase antibody detects O-GlcNAc transferase protein at cytoplasm and nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. O-GlcNAc transferase antibody diluted at 1:500.
Application
Immunoprecipitation of O-GlcNAc transferase protein from A431 whole cell extracts using 5 μg of O-GlcNAc transferase antibody . Western blot analysis was performed using O-GlcNAc transferase antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | 293T , A431 , HeLa , HepG2 , mouse brain , PC-12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
