Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody [N1N2], N-term diluted at 1:1000.Application
O-GlcNAc transferase antibody [N1N2], N-term detects O-GlcNAc transferase protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. O-GlcNAc transferase antibody [N1N2], N-term dilution:Application
O-GlcNAc transferase antibody [N1N2], N-term detects OGT protein at cytosol on U373 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U373 xenograft. O-GlcNAc transferase antibody [N1N2], N-term dilution: 1:500.Application
Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE diluted at 1:1000| Product Name | O-GlcNAc transferase antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to O-GlcNAc transferase (O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase))O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human O-GlcNAc transferase. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Pig, BovineXenopus laevis (100%), Pig (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ23071 antibody HRNT1 antibody MGC22921 antibody O-GLCNAC antibody OGT antibody O-GlcNAc transferase p110 subunit antibody uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase antibody O-GlcNAc transferase subunit |
|---|---|
| Molecular Weight(MW) | 117 kDa (note) (2) |
| Cellular Localization | Cytoplasm (Potential) , Nucleus |
Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody [N1N2], N-term diluted at 1:1000.
Application
O-GlcNAc transferase antibody [N1N2], N-term detects O-GlcNAc transferase protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. O-GlcNAc transferase antibody [N1N2], N-term dilution:
Application
O-GlcNAc transferase antibody [N1N2], N-term detects OGT protein at cytosol on U373 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U373 xenograft. O-GlcNAc transferase antibody [N1N2], N-term dilution: 1:500.
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE diluted at 1:1000| Positive Control | HeLa , mouse brain , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
