| Product Name | Oncostatin M antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [17001.31] to Oncostatin MOncostatin M (OSM) is a growth-regulating cytokine that affects a number of tumor and normal cells. OSM was first identified by its ability to inhibit the growth of A375 melanoma cells and other human tumor cells, but not inhibit the growth of normal human fibroblasts. Recombinant human OSM is produced from a DNA sequence encoding the mature 196 amino acid residue of human OSM. Oncostatin M acts synergistically with TGF-beta-1 to inhibit the proliferation of A375 melanoma cells. Oncostatin M is secreted by macrophages and activated T lymphocytes. It affects a wide variety of normal and tumor cells. It induces an increase in LDL receptor expression and LDL uptake by hepatoma cells. OSM will induce cultured human endothelial cells to increase IL6 production. It activates synovial fibroblast-like cells to produce urokinase type plasminogen activator. Oncostatin M, LIF, GCSF, IL6 and ciliary neurotrophic factor (CNTF) are structurally related members of the same cytokine family sharing similarities in their primary amino acid sequences, predicted secondary structure, and receptor components. |
| Immunogen | Recombinant full length protein (Human) expressed in E. coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 17001.31 |
| Host Species | Mouse |
| Tested Applications | ELISANeutWB |
| ELISA: Use at a concentration of 0.5-1 μg/ml. Neut: ND50 = 0.1 - 5 μg/mlWB: Use at a concentration of 1-2 μg/ml. Predicted molecular weight: 22 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Protein A |
| Gene Synonyms | MGC20461 |
|---|---|
| Target | Oncostatin M |
| Tissue Specificity | shows |
| Cellular Localization | Secreted |
| Positive Control | Tested with human TF-1 cell line. |
|---|---|
| Application Notes | ELISA: Use at a concentration of 0.5-1 μg/ml. Neut: ND50 = 0.1 - 5 μg/mlWB: Use at a concentration of 1-2 μg/ml. Predicted molecular weight: 22 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Constituents: PBS |
