Application
Optineurin antibody detects Optineurin protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Optineurin protein stained by Optineurin antibody diluted at 1:500.Application
Optineurin antibody detects Optineurin protein at cytoplasm in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. Optineurin antibody diluted at 1:500.Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Optineurin antibody diluted at 1:2000.| Product Name | Optineurin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to OptineurinThis gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Optineurin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Pig, Rhesus Monkey, BovinePig (82%), Rhesus Monkey (92%), Bovine (81%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ALS12 antibody FIP2 antibody GLC1E antibody HIP7 antibody HYPL antibody NRP antibody OPTN antibody Optineurin antibody Optineurin antibody TFIIIA INTP antibody |
|---|---|
| Target | Optineurin |
| Molecular Weight(MW) | 66 kDa (note) (4) |
| Cellular Localization | Cytoplasm , perinuclear region , Golgi apparatus |
Application
Optineurin antibody detects Optineurin protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Optineurin protein stained by Optineurin antibody diluted at 1:500.
Application
Optineurin antibody detects Optineurin protein at cytoplasm in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. Optineurin antibody diluted at 1:500.
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Optineurin antibody diluted at 1:2000.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
